Custom Chimeric Gas Vesicle (CGV) Design and Manufacturing

Gasilus offers a comprehensive custom CGV service. Similar to established peptide-conjugation services for immunogens like BSA or KLH conjugates, we streamline CGV creation for your specific needs:

1. Provide your amino acid sequence: Simply send us your desired amino acid sequence (between 8 and 50 amino acids) by email.

2. Plasmid construction: Our team will design and construct the plasmid encoding your chosen sequence fused with the CGV rib protein.

3. Expression and purification: We express and purify the CGVs in our state-of-the-art facilities.

4. Delivery: You'll receive the sterile and high-quality CGV products ready for further development.

Benefits of Gasilus Custom CGV Service:

• Fast and efficient: Get your CGVs quicker in one month with our streamlined process.

• Expert design and construction: Leverage our team's knowledge for optimal CGV development.

• High-quality production: Benefit from our expertise in CGV expression and purification.

• Scalability: We can accommodate your needs, from research-scale to large-scale production.

What CGVs can do:

• Generating pAb with enhanced specificity: CGVs excel as immunogens for generating polyclonal antibodies (pAbs). Because they display specific epitopes, CGVs lead to pAbs with greater focus on the desired target compared to pAbs generated using whole protein immunogens.

• Streamlined hybridoma discovery: The enhanced specificity of CGV-derived pAbs translates to a significant advantage in downstream hybridoma discovery for monoclonal antibody (mAb) generation. By using CGVs as immunogens, you'll likely need to screen far fewer hybridoma clones to identify the desired mAb. This translates to a 5-10 fold reduction in selection effort.

• Promising vaccine platform: Studies in mice have shown that CGVs can be effective vaccines, inducing both mucosal and humoral immune responses. This makes them a promising platform for developing new vaccines.

• Potential for other applications: CGVs may have broader applications beyond antibody discovery and vaccines. They hold potential for use in T cell or B cell separation techniques and phage display, a method for identifying protein-protein interactions.